东海原甲藻的分子鉴定

采用nrDNAITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNAITS序列碱基差异值仅为0.002,nrDNA18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NAITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNAITS更适合于该种属界定的分子标准.     

Analytically derived wind-wave directional spectrum part 1. Derivation of the spectrum

In contrast with the usual method to obtain the wind-wave directional spectrum by multiplying the frequency spectrum with an empirical directional function, the authors attempt to derive analytically the directional spectrum by adopting proper spectral form and using effective parameters, namely, the zero order momentm ₀ of the wind-wave frequency spectrumS(), its peak frequency ₀ and the so-called peakness factorP=₀ S(₀)/m ₀, where is angular frequency. The directional spectrum is given in a form of frequency spectrum for each direction. The spectral directionality depends on, in addition to frequency, the wind-wave growth status, for the peakness factorP as introduced by the authors previously is a measure of the wave development stage. The salient features of the directional spectrum, comparison with existing formulas and the verification of the spectrum by observational data are to be given in the Part 2 of the paper.Project supported by the National Natural Science Foundation of China.     

扇贝提取物对实验性高脂血症大鼠血脂及脂质过氧化的影响

硒与心血管的结构、功能及其疾病的发生关系密切。世界卫生组织和国际原子能协会已把硒列为与冠心病发病有关的５种元素之一而加以研究。近年来 ,人们逐渐认识到脂质过氧化机理在动脉粥样硬化发生发展中的作用 ,体内血脂代谢异常及氧化损伤是动脉粥样硬化发生发展的重要原因。硒作为自由基清除剂在一定程度上 ,有调节血脂代谢及抗氧化的作用。饮食摄入一定量的硒和体内维持一定水平的硒 ,对防止高脂血症具有积极意义。扇贝提取物是以扇贝为原料提取制备的富含微量元素硒及多种微量元素、维生素和蛋白质等生理活性物质的富硒提取物。本研究利…     

Theoretical wind wave frequency spectra in deep water——Ⅰ. Form of spectrum

In this part ot the paper theoretical wind-wave spectra nave been derived oy (I) expressing the spectrum in series composed of exponential terms; (2) assuming that the spectrum satisfies a high order linear ordinary differential equation; (3) introducing proper parameters in the spectrum; and (4) making use of some known charateristics of wind-wave spectrum, for instance, the law governing the equilibrium range. The spectrum obtained contains the zero order moment of the spectrum m0, the peak frequency ω0 and the ratio R =ω/ω0 (ω being the mean zero-crossing frequency) as parameters. The shape of the nondimensional spectrum S(ω) = ω0S(ω)/m0(ω=ω/ω0) changes with R and theoretically reduces to a Dirac delta function δ(ω-1) when R = 1. A spectrum of simplified form is given for practical uses, in which R is replaced by a peakness factor P=S(1).     

Dynamics of subtidal flow in the Taiwan Strait

Two current meter moorings were deployed in the Taiwan Strait from 3 April to 5 May 1983. The data indicate that the subtidal flow in this rather wide strait can be separated into two parts: the mean flow and wind-driven flow, which make about equal contributions to the overall flow. The mean flow is probably originates from branching of the Kuroshio through Bashi Channel. The magnitude of the current is nearly 30 cm sec^–1 flowing northward, and the corresponding along-strait sea surface slope is on the order of 10^–7. The residual flow is a local wind-driven flow, and reaches a frictionally balanced state in about 4 hr. Assuming a linear drag law for the bottom stress, a hindcast scheme is constructed and the results compare well with the observations.     

伶仃洋L＿2和L＿（16）孔第四纪有孔虫群与孢粉化石带特征及其地质意义

本文所依据的分析样品是取自珠江最大的一个河口湾伶仃洋中部的水下钻孔岩芯，其底部到达花岗岩基底。通过对第四纪有孔虫群与孢粉化石带特征的分析，阐明在相同的钻孔岩芯中，有孔虫分布变化所反映的古沉积环境特征与孢粉分析的古气候特征较为吻合，并与相应的沉积相对应，结合￣（１４）Ｃ和￣（２３０）Ｔｈ／￣（２３２）Ｔｈ比值法测年数据，从而较好地重塑本区晚更新世中期以来的地质历史。     

Quantizing and analyzing the feature information of coastal zone based on high-resolution remote sensing image

1Introduction With the development and application of remotesensing technology, people fromthe international re-mote sensing community have realized the issue thatwe have immoderately emphasized characteristics ofvast volume data and great detail of remot…     

中华鲟配合饲料适宜蛋白质含量及最佳蛋白能量比研究

以酪蛋白和明胶为蛋白源 ,添加豆油、鱼油、糊精、纤维素以及适量的维生素和矿物质混合剂配制试验饲料 ,饲料配成5个蛋白质水平 ,每个蛋白水平分别包括5个能量级 ,以体质量增长倍数、蛋白质效率和饲料系数为评判依据 ,对体质量为8.73g±0.79g的中华鲟(Acipensersinensis)的适宜蛋白质含量及其蛋白能量比进行了研究。经过为期8周的试验 ,结果表明 :中华鲟幼鲟配合饲料中的适宜蛋白质含量范围为35.7%～41.8% ,饲料的最佳蛋白能量比范围为21.85～30.05ng/J。当试验饲料蛋白质含量为40.3% ,蛋白能量比为27.85ng/J时 ,试验鲟鱼获得最大体质量增长倍数为11.60±1.20,最低的饲料系数为1.68±0.23,以及最高的蛋白质效率为1.58%±0.14%。     

凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。     

Cu2+、Zn2+、SDS、DBS对脊尾白虾的毒性试验

选用铜、锌2种常见重金属和十二烷基硫酸钠(SDS)、十二烷基苯磺酸钠(DBS)2种阴离子表面活性剂对脊尾白虾Palaemon carincauda仔虾进行96h急性毒性试验。结果表明,Cu2 、Zn2 、SDS、DBS对脊尾白虾仔虾的96h LC50分别为0.34、0.82、14.3和14.4 mg.L-1。将脊尾白虾仔虾对这4种毒物的敏感性与文献报道的其它动物进行了比较,结果认为可以将其作为毒性测试的标准试验生物。